Journal: JACC: Basic to Translational Science
Article Title: Periodontitis Accelerates Progression of Heart Failure With Preserved Ejection Fraction in Mice
doi: 10.1016/j.jacbts.2025.03.002
Figure Lengend Snippet: Effects of PD on Myocardial Protein Expression and Activity of Signal Pathways Involved in HFpEF Pathogenesis (A) Bar chart representing cyclic guanosine monophosphate (cGMP) quantitative analyses of myocardial tissue homogenates obtained from the different mice groups as assessed by enzyme-linked immunosorbent assay. n = 5-8 mice per group. (B) Representative western blot films. (C) Protein kinase G (PKG) expression levels normalized to actin. n = 5-9 mice per group. (D) PI3K expression levels normalized to actin. n = 6-8 mice per group. (E) p-AKT/AKT ratio. Both p-AKT and AKT were normalized to their actin separately. n = 4-10 mice per group. Data are presented as mean ± SEM. 1-way analysis of variance followed by Tukey’s multiple-comparisons test was conducted. Abbreviations as in .
Article Snippet: Proteins were detected by incubating the membranes with primary antibodies against AKT1/2/3 isoforms [ EPR16798 ] (ab179463, Abcam), phospho-AKT/1/2/3 (phospho-S472/473/474) [ EPR18853 ] (ab192623, Abcam), phosphoinositide 3 Kinase (PI3K) p85 alpha [ EPR18702 ] (ab191606, Abcam), PKG-1α (13511, Cell Signaling Technology), or the housekeeping protein actin (08691001, MPB).
Techniques: Expressing, Activity Assay, Enzyme-linked Immunosorbent Assay, Western Blot